Journal: PLOS ONE
Article Title: Rapid and specific detection of wheat spindle streak mosaic virus using RT-LAMP in durum wheat crude leaf extract
doi: 10.1371/journal.pone.0299078
Figure Lengend Snippet: The test was run on RNA from samples infected with WSSMV CAD22-W1 (positive control), WSSMV DSMZ PV-0541, SBCMV and SBWMV, all diluted 10 −3 . Negative control: healthy plant; Water control: No-template control; the bCUBE instrument was used, with three technical replicates. Some lines are not visible in the graph because the fluorescence they produced did not exceed that of the no-template control.
Article Snippet: Analytical specificity, i.e. the ability of the RT-LAMP to detect the target viral sequences rather than others, was tested on total RNA obtained from plants infected by the target organism WSSMV (CAD22-W1 Italian isolate and DSMZ PV-0541 German isolate; GenBank OP357940), and from plants infected by the non-target organisms SBCMV (PLAVIT-CAD22 isolate from Italy) and SBWMV (provided by Dr. A. Niehl, Julius Kühn-Institut, Germany).
Techniques: Infection, Positive Control, Negative Control, Control, Fluorescence, Produced